Proinflammatory genes as biomarkers and therapeutic targets in oral squamous cell carcinoma. In parallel, we also determined the populations colony forming units cfusml, which provides an estimate of the viable cells, by plating serial dilutions of the bacterial cultures on tryptic soy agar tsa, difco. A gallic acid standard curve was used to estimate total phenolic content of each extract, expressed as mg of gallic acid equivalents per gram of extract. Ge healthcare amersham biosciences ultrospec 3100 pro. Ge healthcare life sciences now the only difficult thing. Ultrospec 2100 pro 3 nm bandwidth instrument, wavelength range 190 to 900 nm with 8position cell changer and. Ultrospec 2100 pro eighteen methods can be saved in three groups of six, making the instrument very practical in a multiuser laboratory. S2000p serial printer includes serial cable spreadsheet interface software serial. Ultrospec 7000 highperformance dualbeam, 2 nm bandwidth uvvisible spectrophotometer. Supporting information porphyrinic metalorganic framework. Nanovue plus data file biochrom spectrophotometers. Ultrospec 2100 pro 3 nm bandwidth instrument, wavelength range 190to 900 nm with 8position cell. Ultrospec 2100 uvvisible spectrophotometer is a simple to use instrument with advanced performance. Biochrom 80211221 ultrospec 2100 pro, classic currently unavailable.
Ultrospec 2100 pro with portable pc, running swift ii software, mounted on pc support plinth. Ease of operation is achieved by using a microprocessor to perform most of the repetitive talks usually requiring prolonged operator action. Recommended for nucleic acid quantification, protein assay, do600nm cell culture single beam visible uv spectrophotometer storage of data and applications. The cell viability was calculated with the percentage of absorbance difference between treated and control samples. The ge healthcare amersham biosciences ultrospec 3100 pro uvvisible spectrophotometer is the ideal instrument for the modern, yet demanding, life science laboratory where bench space is at a premium. Optional resolution pc software for instrument control and extended analysis options. Versatility of application is provided by four builtin operational modes, a wide range of accessory cells and cell holders, and the possibility of adding a personal computer supported with lkb application software available from lkb. New listing pharmacia biotech ultrospec 3000 uvvis spectrophotometer.
Data export options includemicrosoft word and excel plus adobe pdf formats. Pseudomonas deceptionensis dc5mediated synthesis of. Single beam visible spectrophotometer wpc scanning software3201100nm. Uvvis spectroscopy was done using an ultrospec 2100 pro uvvisible spectrophotometer biochrom, uk at wavelengths ranging from 200 to 800 nm. Ultrospec 2100 pro 3 nm bandwidth instrument, wavelength range. Ultraviolet uv analysis was on ultrospec 2100 pro uvvisible spectrophotometer biochrom with swift ii software amersham. Recommended for life science applications, with optional life science or cfr datrys software upgrades.
In addition to the basic modes of operation, the instrument has enhanced software and. Silver nanoparticles synthesized biogenically from aloe. Proinflammatory genes as biomarkers and therapeutic. With a range of output modes direct to microsofttm excel, control by swift ii software, output to seiko dpu414 printer and self test diagnostics for glp purposes, the ultrospec 2100 pro is an excellent. Ultrospec 7000 high performance doublebeam, 2 nm bandwidth uvvisible spectrophotometer. In addition to measuring absorbance and concentration, it provides stored routines for nucleic acid quantization and also a standard curve routine for protein determination. Each sample was incubated for 2 h at room temperature and the absorbance at 765 nm was measured by using a uvvis spectrophotometer ultrospec 2100 pro, amersham biosciencesge healthcare. Determination of antioxidant activity in garlic allium.
An initial reference is required to standardise the instrument before analysis can begin. Pc12 cells were cultured in medium containing 15% hs, 2. To check the genomic dna contamination, we loaded the total rnas in a 1% agarose gel that contained ethidium bromide etbr and the gel was visualized on a uv transilluminator wealtec corp. Ultrospec 2100 pro uvvisible spectrophotometer is a simple to use instrument with advanced performance. Ultrospec 2100 pro spectrophotometer spectrophotometers. Image analyses were performed automatically from the shapes of 4. Oxidizing and nanodispersing the natural silk fibers. Adhesive tablet effective for treating canker sores in humans. Results may then be formatted or manipulated as appropriate prior to. L distilled water droplets dropped onto the membranes within 0. Interface software installed 80 211073 and the two are linked with the serial cable. Subsequently, we loaded the total rna in a 1% formaldehydeagarose gel with etbr. Compatible with ultrospec 2100 pro and ultrospec 3100 pro spectrophotometers. Ultrospec 2100 pro 3 nm bandwidth instrument, wavelength range 190.
Domb 1department of medicinal chemistry and natural products, school of pharmacy, the hebrew university of jerusalem, jerusalem 91120, israel 2department of parasitology, school of medicine, the hebrew university of jerusalem, jerusalem, israel. Consult ge healthcare life sciencess entire ultrospec 2100 pro. Single beam refers to the fact that the light just passes through the sample holder straight to the detector. The amersham biosciences ge healthcare ultrospec 2100 pro is a uvvis spectrophotometer. Datrys lite datrys lite offers control of the basic functions for quick read and fast scanning. This is essential information for optimizing the concentration of each labeled probe before use in. Single beam instruments are generally very simple and economical to purchase. Optional life science and cfr datrys software upgrades. It is available in a range of colors classic, yellow, plum, apple and has both exciting styling. Interface software installed 80211073 and the two are linked with the serial cable. Amersham biosciences ge healthcare ultrospec 2100 pro.
Grafico pc utilit software, serial cable and dust cover. Pharmacia lkb biochrom ultrospec ii is designed as a simple to use instruments capable of producing rapid measurement of light absorption or transmission. The relevant software module for the application being carried out is selected from. Lkb pharmacia 4050 ultrospec ii uvvis spectrophotomete ebay. View online or download ge ultrospec 8000 original instructions manual. Further analysis was performed using genespringgx10.
Ultrospec 3100 pro uvvisible spectrophotometer ge healthcare. Biochrom ultrospec 2100 pro uvvis spectrophotometers fisher. Optional life science datrys software upgrade available. User manuals, ge measuring instruments operating guides and service manuals. Optional life science and cfr datrys software upgrades available. Description the ultrospec 2100 pro uvvisible spectrophotometer has an integral high resolution graphics display and easytouse software making it ideal for use in the modern laboratory. Ultrospec 700080009000 instruments have the onoff switch on the left hand side of the instrument. Manufacturer specifications ultrospec, pharmacia biotech please note. According to the variation of absorbance a with the concentration c of standard solution. Prolonged exposure to alcohol, tobacco, and pathogenic agents are known risk factors and have suggested that chronic inflammation may represent a potential common denominator in the development of oscc. Fourier transform infrared ftir spectroscopy ftir spectroscopy analysis was carried out for both agnps and extract of a. Th e xray diff raction xrd analyses were performed using an xray diff ractometer d8 advance, bruker, germany, operated at 40 kv, 40 ma, with cuk.
None of the equipment posted for sale on is owned by medwow, should you have any questions regarding a specific item, please direct them to the appropriate seller by making use of the available communication channels on the items page. Nanovue, ultrospec 2100 pro, ultrospec 7000 upgrade for. Ultrospec 2100310053006300 units have an onoff switch on the back of the instrument on the right hand side. Recommended for nucleic acid quantification, protein assay, do600nm cell culture single beam visible uv spectrophotometer storage of data and applications on usb key, export at will to the pc allows up to 18 users pcan be controlled. Ge measuring instruments user manuals download manualslib. The ultrospec 1100 pro uvvisible spectrophotometer is an easy to use instrument that is optimized for the biological science teaching laboratory and for industrial, utility qc, and environmental testing situations. The ultrospec 2100 pro uvvisible spectrophotometer has an integral high resolution graphics display and easytouse software making it. Ultrospec 2100 pro 3 nm bandwidth instrument, wavelength range 190 to 900 nm. Adhesive tablet effective for treating canker sores in humans boaz mizrahi, 1jacob golenser,2 joseph s. Scan comprising data may be picked up as columns of numbers and converted to a more conventional graphical form using the spreadsheet. In addition to measuring absorbance and concentration, it provides stored routines for nucleic acid quantitation and also a standard curve routine for protein determination. Ultrospec 2000 has almost the same codes as ultrospec 3000.
Alkylation of thiol groups all the buffers used in this study are analytical grade products from tianjin fengchuan chemical reagent technologies co. Consult ge healthcare life sciencess ultrospec 10 cell density meter brochure on medicalexpo. Biochrom spreadsheet interface software for use with. For conversion data for nucleic acids and proteins, see the technical appendix.